But up to today, the technique is far from perfection because of the low blastocyst production rate and the poor quality of the blastocyst .
但是到目前为止体外培养的哺乳动物早期胚胎，高质量的囊胚发育率还不高，都还远远不能满足人们生产和研究的需求。Morulae and blastocyst came into a period of rapid growth and differentiation.
桑椹胚囊胚期胚胎进入快速生长分化时期。From two variants of the experiment the most sensitive was the second one, in which polysaccharides were added at the late blastula stage.
从两个实验中，最敏感的是第二个变种，其中多糖在晚囊胚期补充。The artificial shrinkage prior to vitrification could improve the survival rate and hatching rate of the vitrified expanded blastocyst.
冷冻前人工皱缩囊腔体积能明显提高扩张囊胚玻璃化冷冻的效率。But recent reports show that the metastatic phenotype of tumor cells can be reversed to normalcy after injecting to the blastocyst.
但是最近研究证明注入早期囊胚后癌细胞的恶性表型可以被逆转为正常状态。The aim of this article is to summarize the influential factors of vitrification and the advance in blastocyst vitrification .
本文就玻璃化冷冻的影响因素和囊胚玻璃化冷冻进展综述如下。The fluid in the cavity must be under positive pressure as this pressure is the motive force for expansion of the blastocyst.
腔中的液体必须处于正压下，因为只有正压才可以促使囊胚的扩张。The technique, called blastocyst complementation, provides us with a novel approach for organ supply.
这项被称为囊胚互补的技术，为我们提供了一种器官供应的新途径。blastula Stage in embryonic development which represents the final product of cleavage stages, characterized by formation of the blastocoel.
囊胚代表胚胎发展的最后分裂阶段，特徵为囊胚腔的形成。